复方苦芩对细小病毒感染犬血液T细胞亚群和细胞因子的影响

doi:10.11843/j.issn.0366-6964.2017.02.021

摘要/Abstract

摘要：

研究复方苦芩对细小病毒感染犬T细胞亚群和细胞因子的影响。建立犬细小病毒感染模型，把动物随机分为6个组，分别为空白对照组，模型组，阳性药物组，复方苦芩高、中、低剂量组，每组20只。将攻毒后6 h设定为试验0 d，分别于试验0、7、14、21、28、35 d采血，流式细胞术检测T淋巴细胞亚群，试剂盒检测IL-2、IL-4、IFN-γ含量。结果显示：与模型组相比，复方苦芩可促进犬机体分泌IL-2、IL-4和IFN-γ，提高外周血CD3⁺T、CD3⁺CD4⁺T细胞百分数，降低CD3⁺CD8⁺T细胞百分数。复方苦芩能够调节细小病毒感染犬的免疫机能，提高犬抗病毒能力。

Abstract:

In order to study the effect of compound Kuqin on T cell subsets and cytokines of blood in canines with canine parvovirus, we established the canine parvovirus model, and separate 120 canines into 6 groups, like blank control group, model group, positive medicine group, compound Kuqin high dose group, middle dose group, low dose group, with 20 canines in each group. We set the time point that 6 hours after the virus was inoculated as 0 d, and collect the blood sampling from the canine saphenous vein at the day 0, day 7, day 14, day 21, day 28, and day 35. T lymphocyte subsets were detected by flow cytometry, and the cytokines IL-2, IL-4, IFN-γ were detected by kit. The result showed that, compared with the model group, compound Kuqin can promote the secretion of IL-2, IL-4, IFN-γ, and increase the percentage of CD3⁺, CD3⁺CD4⁺T cells in peripheral blood, and decrease the percentage of CD3⁺CD8⁺T cells. In conclusion, compound Kuqin can regulate canines’ immune function and improve the ability of antiviral after they were infected by canine parvovirus.

中图分类号:

S853.74

刘娟，吴秋月，邱洪. 复方苦芩对细小病毒感染犬血液T细胞亚群和细胞因子的影响[J]. 畜牧兽医学报, 2017, 48(2): 369-376.

LIU Juan, WU Qiu-yue, QIU Hong. Effect of Compound Kuqin on T Cell Subsets and Cytokines of Blood in Canines with Canine Parvovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 369-376.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2017.02.021

https://www.xmsyxb.com/CN/Y2017/V48/I2/369

参考文献

［1］颜文卿, 吴德峰, 戴亚东, 等. 犬细小病毒病的病原学研究进展［J］. 动物医学进展, 2006, 27(1): 48-51.
YAN W Q, WU D F, DAI Y D, et al. Study on the pathogeny of canine parvovirus［J］.Progress in Veterinary Medicine,2006,27(1):48-51.(in Chinese)
［2］艾静, 袁玉国, 彭秋玲, 等. 犬细小病毒病的流行病学调查［J］. 中国畜牧兽医, 2012, 39(11): 200-202.
AI J, YUAN Y G, PENG Q L, et al. Epidemiological investigation of canine parvovirus［J］.China Animal Husbandry & Veterinary Medicine,2012,39(11):200-202.(in Chinese)
［3］顾海军, 汪建亚, 王军, 等. 犬细小病毒病的防治与体会［J］. 中国动物保健, 2010(4): 100-102.
GU H J, WANG J Y, WANG J, et al. Prevention and treatment of canine parvovirus ［J］. China Animal Health,2010(4): 100-102.(in Chinese)
［4］李明霞, 王伟. 犬细小病毒病的诊断与综合防治［J］. 吉林畜牧兽医, 2013(2): 58-59.
LI M X, WANG W. Diagnosis and comprehensive prevention and treatment of canine parvovirus［J］. Jilin Animal Husbandry and Veterinary Medicine,2013(2): 58-59.(in Chinese)
［5］HOARE C M, DEBOUCK P, WISEMAN A. Immunogenicity of a low-passage, high-titer modified live canine parvovirus vaccine in pups with maternally derived antibodies［J］. Vaccine, 1997, 15(3): 273-275.
［6］JIANG W, BAKER H J, SWANGO L J, et al. Nucleic acid immunization protects dogs against challenge with virulent canine parvovirus［J］. Vaccine, 1998, 16(6): 601-607.
［7］MORRISON M D, REID D, ONIONS D, et al. Generation of E3-deleted canine adenoviruses expressing canine parvovirus capsid by homologous recombination in bacteria［J］. Virology, 2002, 293(1): 26-30.
［8］汪恭富, 杨沈, 朱燕, 等. 犬细小病毒单克隆抗体在犬细小病毒病治疗中的应用［J］. 中国兽医杂志, 2007, 43(3): 46.
WANG G F,YANG S, ZHU Y, et al. Application of monoclonal antibodies to canine parvovirus ［J］.Chinese Journal of Veterinary Medicine, 2007, 43(3): 46.(in Chinese)
［9］IKEDA Y, MOCHIZUKI M, NAITO R, et al. Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats［J］. Virology, 2000, 278(1): 13-19.
［10］STEINEL A, MUNSON L, VAN VUUREN M, et al. Genetic characterization of feline parvovirus sequences from various carnivores［J］.J Gen Virol, 2000, 81(Pt 2): 345-350.
［11］POLLOCK R V, CARMICHAEL L E. Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination［J］.J Am Vet Med Assoc, 1982, 180(1): 37-42.
［12］O'BRIEN S E, ROTH J A, HILL B L. Response of pups to modified-live canine parvovirus component in a combination vaccine［J］. J Am Vet Med Assoc, 1986, 188(7): 699-701.
［13］朱兆荣, 刘娟, 聂晨睿. 复方苦芩注射液对人工感染犬细小病毒病的防治［J］. 中兽医医药杂志, 2010(6): 34-37.
ZHU Z R, LIU J, NIE C R. Curative and preventive effects of compound Kuqin injection against canine Parvovirus disease in dogs［J］. Journal of Traditional Chinese Veterinary Medicine,2010(6): 34-37.(in Chinese)
［14］王学林, 刘文森, 王承宇. 抗犬细小病毒复方制剂的研究［J］. 畜牧与兽医, 2004, 36(4): 7-8.
WANG X L, LIU W S, WANG C Y. Study on anti-CPV composite［J］. Animal Husbandry & Veterinary Medicine,2004, 36(4): 7-8.(in Chinese)
［15］聂晨睿. 复方苦芩对犬细小病毒病防治作用及药理研究［D］.重庆：西南大学,2008.
NIE C R. Research on the preventive and therapeutic effect of complex prescription Kuqin on CPV and pharmacodynamic action［D］.Chongqing: Southwest University,2008.(in Chinese)
［16］杨光维, 李润楠, 于为力. 利用3种中药组方对比治疗犬细小病毒病的效果观察［J］. 中国兽医杂志, 2007, 43(9): 58-59.
YANG G W, LI R N, YU W L. Observation on effect of three kinds of traditional Chinese medicine in treating canine parvovirus disease［J］.Chinese Journal of Veterinary Medicine,2007, 43(9): 58-59.(in Chinese)
［17］尹彦军. 中药 “犬康” 治疗犬细小病毒性肠炎试验［J］. 中国动物检疫, 2002, 19(3): 27-28.
YIN Y J. Treatment of canine parvovirus enteritis with traditional Chinese medicine “Quan Kang”［J］. Chinese Journal of Animal Quarantine, 2002, 19(3): 27-28.(in Chinese)
［18］刘钟瑸, 王雅，胡杨. T 细胞亚群和细胞因子在抗感染免疫中的作用［J］. 国外医学免疫学分册, 1992(4): 206-208,224.
LIU Z B, WANG Y, HU Y. Role of T cell subsets and cytokines in anti-infection immunity［J］. International Journal of Immunology,1992(4): 206-208,224. (in Chinese)
［19］诸葛证，朱兆荣，刘娟. 复方苦芩对细小病毒病犬红细胞及血红蛋白变化的影响［J］. 内江科技， 2009, 30(12): 25.
ZHUGE Z, ZHU Z R, LIU J. Effect of Kuqin compound on canine parvovirus of red blood cell and hemoglobin changes［J］.Neijiang Science and Technology,2009, 30(12): 25.(in Chinese)
［20］北京农业大学.兽医学［M］.北京：农业出版社,1999.
Beijing Agricultural University.Veterinary medicine［M］.Beijing: Agriculture Press,1999.(in Chinese)
［21］孔庆波, 陈进军, 陈德坤, 等. 两种转移因子对犬外周血 T 细胞亚群的影响［J］. 中国畜牧兽医, 2007, 34(7): 56-59.
KONG Q B, CHEN J J, CHEN D K, et al. Effects of two transfer factors on T-Lymphocytic subgroups in canine peripheral blood［J］.China Animal Husbandry & Veterinary Medicine,2007, 34(7): 56-59.(in Chinese)
［22］盛鹏飞, 蒋月. 中西药结合治疗藏獒犬细小病毒病［J］. 畜牧兽医科技信息, 2008(5): 105.
SHENG P F, JIANG Y. Treatment of tibetan mastiff parvovirus by combination of traditional Chinese medicines［J］.Chinese Journal of Animal Husbandry and Veterinary Medicine,2008(5): 105.(in Chinese)
［23］景秀年, 李中利, 杨小华, 等. 犬细小病毒的中医治疗［J］. 四川畜牧兽医, 2002, 29(3): 48.
JING X N, LI Z L, YANG X H, et al.Treatment of canine parvovirus by traditional Chinese medicine［J］.Sichuan Animal and Veterinary Sciences, 2002, 29(3): 48.(in Chinese)
［24］杨汉春,姚火春,王君伟,等. 动物免疫学［M］.北京：中国农业大学出版社,2003.
YANG H C, YAO H C, WANG J W, et al. Immunology［M］.Beijing:China Agricultural University Press,2003.(in Chinese)
［25］NG C T, SNELL L M, BROOKS D G, et al. Networking at the level of host immunity: immune cell interactions during persistent viral infections［J］. Cell Host Microbe, 2013, 13(6): 652-664.
［26］WHERRY E J, KURACHI M. Molecular and cellular insights into T cell exhaustion［J］. Nat Rev Immunol, 2015, 15(8): 486-499.
［27］PAUL W E, SEDER R A. Lymphocyte responses and cytokines ［J］. Cell, 1994, 76(2): 241-251.
［28］TANIGUCHI T, MINAMI Y. The IL-2/IL-2 receptor system: a current overview［J］. Cell, 1993, 73(1): 5-8.
［29］侯健,王东星,胥军民,等. 人类细胞因子手册［M］. 上海: 同济大学出版社, 1996.
HOU J, WANG D X, XU J M, et al. A handbook of human cytokines［M］.Shanghai: Tongji University Press,1996.(in Chinese)
［30］刘志超,岳屹囡. 细胞因子与临床疾病［M］. 海口: 海南出版社, 2008.
LIU Z C, YUE Y N.Cytokines and clinical disease［M］.Haikou: Hainan Press, 2008.(in Chinese)
［31］万遂如,康丽娟. 细胞因子在畜禽疫病防控中的科学应用［M］. 北京: 中国农业出版社, 2010.
WAN S R, KANG L J. Application of cytokines in the prevention and control of animal diseases［M］.Beijing: China Agricultural University,2010.(in Chinese)
［32］BROWN M A, HURAL J. Functions of IL-4 and control of its expression［J］. Crit Rev Immunol, 1997, 17(1):1-32.
［33］LEVY D E, GARCA-SASTRE A. The virus battles: IFN induction of the antiviral state and mechanisms of viral evasion［J］. Cytokine Growth Factor Rev, 2001, 12(2-3): 143-156.

[1]	薛霖莉, 孙睿, 郝晓静, 曹校瑞, 王海东, 卢嘉茵. 基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用[J]. 畜牧兽医学报, 2023, 54(12): 5252-5263.
[2]	池幸子, 李耀星, 王慧婷, 严铭恩, 杨诗靖, 杨泊文, 孙晗, 郭世宁, 石达友, 武力, 刘翠. 紫锥菊提取物联合柳氮磺吡啶对湿热泄泻大鼠Th17/Treg免疫失衡的影响[J]. 畜牧兽医学报, 2023, 54(11): 4817-4826.
[3]	张旭梅, 魏玉荣, 许丞惠, 杨彤, 史慧君, 付强, 杨莉. 基于网络药理学和试验验证分析小檗碱治疗鸡沙门菌感染的作用机制[J]. 畜牧兽医学报, 2023, 54(8): 3557-3570.
[4]	巩志国, 赵佳敏, 顾柏臣, 任佩佩, 于琢雅, 白云洁, 刘鑫煜, 王超, 刘博. 基于网络药理学分析党参减轻大肠杆菌感染小鼠急性肺损伤的作用机制[J]. 畜牧兽医学报, 2023, 54(8): 3571-3581.
[5]	潘婵媛, 赵梓轩, 段铭洁, 蒋林树, 童津津. 基于网络药理学预测青蒿缓解奶牛氧化应激的作用机制[J]. 畜牧兽医学报, 2023, 54(3): 1071-1084.
[6]	常颖艳, 赵鸿雁, 胡茂志, 刘宗平. 枸杞酰胺体外对镉致成骨细胞凋亡的保护作用[J]. 畜牧兽医学报, 2023, 54(3): 1273-1280.
[7]	刘广, 龚成燕, 牛凯敏, 张硕, 陶灯, 马军, 吴信, 印遇龙, 唐宇龙. 连花清瘟药渣及发酵产物的生物活性研究[J]. 畜牧兽医学报, 2023, 54(3): 1281-1299.
[8]	霍相羽, 高家瑞, 靳佳祺, 李燕, 高原, 刘梦涵, 蒋林树, 童津津. 苦参碱对牛源无乳链球菌的体外抑菌活性分析[J]. 畜牧兽医学报, 2023, 54(3): 1300-1309.
[9]	张文嫦, 王志华, 练家乐, 屈倩, 吕伟杰, 陈淑爱, 郭世宁. 仔鼠哺乳期补充参苓白术散改善抗生素诱导的肠道稳态失衡[J]. 畜牧兽医学报, 2023, 54(2): 825-836.
[10]	马天文, 于跃, 吕良钰, 贾丽娜, 阮红日, 王颢然, 王新宇, 张雨欣, 张建涛, 高利. 白果内酯对IL-1β诱导的ATDC5软骨细胞自噬、增殖和凋亡的影响[J]. 畜牧兽医学报, 2023, 54(2): 837-846.
[11]	池幸子, 杨诗靖, 杨泊文, 孙晗, 姚丽丽, 顾达星, 郭世宁, 石达友, 武力, 刘翠. 紫锥菊提取物对大鼠湿热泄泻的疗效及其机制[J]. 畜牧兽医学报, 2023, 54(1): 380-391.
[12]	未张怡, 韩飞, 范婷婷, 陈子贤, 李寒梅, 袁非凡, 丛日华, 李贤. 苦参苍术颗粒对大肠杆菌感染肉鸡肝组织胆固醇代谢的调节作用[J]. 畜牧兽医学报, 2023, 54(1): 392-402.
[13]	崔恩慧, 薛玉环, 李辞霞, 王帅, 朱晓岩, 柴学军, 赵善廷. 杜仲叶免疫调节机制的网络药理学分析及验证[J]. 畜牧兽医学报, 2023, 54(1): 403-413.
[14]	刘贺娟, 史晨曦, 王静, 王美乐, 王栋涵, 魏战勇, 尹素改. 基于网络药理学探讨黄芩素对猪丁型冠状病毒感染的潜在作用机制[J]. 畜牧兽医学报, 2022, 53(11): 4097-4109.
[15]	王乐, 陈泓岑, 张永红, 吴琼, 侯佳佳, 王天祎, 卢天航, 黄传发, 张华, 崔德凤. 基于网络药理学联合16S rDNA高通量测序技术分析丹参对感染大肠杆菌小鼠肠道菌群的影响[J]. 畜牧兽医学报, 2022, 53(10): 3695-3711.